S. pombe strains
WebAbstract. The Schizosaccharomyces pombe genome is one of the smallest among the free-living eukaryotes. We further reduced the S.pombe gene number by large-scale gene … Web10 Mar 2016 · We used the engineered S. pombe strains to establish a proof of principle whole-cell biosensor by utilizing the secreted peptide pheromone to create a read-out signal. To this end, the nmt1 promoter of S. pombe, which can be tightly regulated by the thiamine (vitamin B1) concentration of the extracellular environment [ 85 ], was used.
S. pombe strains
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Web21 Mar 2024 · The between-strain genomic divergence measured by single nucleotide variants ranges from 0.06 to 0.6%, with a mean of 0.36%. For each strain, we collected approximately 1–3 million colonies to... WebThe fission yeast, Schizosaccharomyces pombe, possesses a UV-damaged DNA endonuclease-dependent excision repair (UVER) pathway in addition to nucleotide excision repair pathway for UV-induced DNA damage. We examined cyclobutane pyrimidine dimer removal from themyo2 locus on the nuclear genome and the coIlocus on the …
WebS. pombe has two mating types, plus and minus, which secrete two different pheromones, P-factor (unmodified) and M-factor (modified), respectively. These pheromones are specifically recognized... WebStrains and media. Cells were grown in yeast extract supplemented with adenine (YES). Standard genetic techniques were used for crossing of strains. S. pombe strains used are listed in Supplementary Table S1. Expression profiling DNA microarrays. Microarrays were purchased from Eurogentec and experiments were carried out as described (Xue et al ...
Web10 Jul 2010 · The preparation of media and basic manipulation methods of S. pombe were carried out as described [ 33 ]. Fission yeast strain NCYC2036 [ 34] with the genotype h - ura4.dl18 was used for transformation by pJMN6. Transformation was done by the lithium acetate method [ 35] to yield the new strain JMN6.
Web3 Aug 1999 · S. pombe AC1 was grown in YPD medium aerobically at 32°C, and exponentially growing cells (10 6 to 10 7 cells per ml) were washed and suspended in …
WebFirst, to see whether S. pombe might provide a good model for functional studies of the Mediator and ELL/EAF interaction, I carried out a thorough proteomic analysis of S. … atenuador padhttp://bahlerweb.cs.ucl.ac.uk/docs/SupplTable7.pdf atentukumpoWebS pombe strains and growth conditions. In document The fission yeast non-coding transcriptome (Page 45-58) All strains used for RNA-seq in the current study are detailed … atenuador hantek 1008cWebTreatment of S. pombe wild-type cells with trabectedin led to cell cycle delay and activation of the DNA damage checkpoint, ... an S. pombe strain carrying a … atenuamWebThese findings indicate that the use of certain S. pombe strains could be advantageous for winemaking in regions where malic acid is problematic, and these strains also show … atenuante da penaWeb24 Jan 2024 · We establish a high-quality curated catalogue of SVs in the genomes of a worldwide library of S. pombe strains, including duplications, deletions, inversions and translocations. We show that copy... atenuantesWebThe mating type locus mat1 of S. pombe has three possible alleles: h+ (plus), h- (minus), and h90 (these should normally be written h+,h-, and h90, but that's harder to read on the web). … atenuar