Dh5 alpha subcloning efficiency
WebDescription. Subcloning Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that provide a transformation efficiency of > 1 x 10 6 cfu/μg … WebIntact Genomics 5-alpha electrocompetent E. coli cells are suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, cloning, and subcloning. Mutations in endA1 and recA1 ensure increased plasmid yield and improved plasmid quality.
Dh5 alpha subcloning efficiency
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WebNEB 5-alpha Competent E. coli (High Efficiency) C2987: 6 x 0.2ml: Subcloning Efficiency ™ DH5α ™ Competent Cells* ThermoFisher (Invitrogen) 18265-017: 4 x 0.5ml: NEB 5-alpha Competent E. coli (Subcloning Efficiency) C2988: 6 x 0.4ml: MAX Efficiency ® DH5αF'IQ ™ Competent Cells* ThermoFisher (Invitrogen) 18288-019: 5 x 0.2ml WebDH5-alpha Chemically Competent E. coli cells are suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, …
WebNEB 5-alpha Competent E. coli (High Efficiency) C2987: 6 x 0.2ml: Subcloning Efficiency ™ DH5α ™ Competent Cells* ThermoFisher (Invitrogen) 18265-017: 4 x 0.5ml: NEB 5 … WebE. coli. (High Efficiency) NEB 5-alpha Competent E. coli is a derivative of the popular DH5α. It is T1 phage resistant and endA deficient for high-quality plasmid preparations. High efficiency strain ideal for a wide variety of applications. Available in a wide variety of sizes, including single-use vials, 200 μl vials, 96- and 384- well ...
WebEconomical for routine subcloning procedures or applications where starting DNA is not limiting. Manufacturer: Invitrogen™ 18265017. Catalog No. 18-265-017. $101.00 / Each …
WebE. coli. (High Efficiency) NEB 5-alpha Competent E. coli is a derivative of the popular DH5α. It is T1 phage resistant and endA deficient for high-quality plasmid preparations. High efficiency strain ideal for a wide …
WebThermo Scientific™ DH5α Competent Cells. Chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of cDNA libraries. Brand: Thermo Scientific™ EC0111. Additional Details : Weight : 17.00000kg. Product Code. 16506900. £50.25 / 40 reactions. how much protein in extra firm tofuWebStandard efficiency (propagation) Subcloning Efficiency™ DH5α™9 (1 x 106 cfu/µg) gDNA or library production One Shot™ OmniMAX 2 T1R (>20 kb) ElectroMAX ™ DH10BOne Shot (>20 kb) ElectroMAX ™ DH10B T1R (>20 kb) MegaX DH10B ™ T1R Electrocomp ™8 (>100 kb) High transformation efficiency (cloning) One Shot ™ … how much protein in fava beansWebSep 8, 2015 · To determine the efficiency and fidelity of DH5α-mediated in vivo assembly and cloning, we designed a pUC19 plasmid-based screen . In this work, efficiency is … how do online magazines make moneyWebThermo Scientific DH5α Competent Cells for Subcloning are chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of … how do online lab classes workWebAug 2, 2024 · This product has been discontinued. Alternatives are NEB® 10-beta Electrocompetent E. coli ( NEB #C3020K) or chemically competent cells, NEB® 5-alpha Competent E. coli (High Efficiency) ( NEB #C2987) Catalog # C2989 was discontinued on August 02, 2024. NEB 5-alpha Electrocompetent Competent E. coli is a derivative of the … how do online petitions workWebtransformation efficiency. The stock solution of pFastBac™-gus (0.2 g/ml), µ provided with pFastBac™1 Expression Vector (Cat. No. 10360-014), can be used as a control for the transposition frequency. To obtain maximum transformation efficiency, the experimental DNA must be free of phenol, ethanol, protein and detergents. 1. how do online savings accounts workWebSubcloning Efficiency™ DH5α™ Competent Cells are tested for transformation efficiency using the pUC19 control DNA supplied with the kit and using the protocol on page 2. Transformation efficiency should be greater than 1 x 106 transformants/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity, how do online hearing tests work